personal_ws-1.1 en 60 dada py TSV tsv demultiplex Jaccard qiime url ASV OTUs cli saveas dev metadata filepath clade demultiplexed Miniconda subsample phylogenetic wget genotype informatics dir qza subsampled conda denoising microbiome confounders covariate Orchanian PairedEndSequencesWithQuality PCoA qzv MiSeq Illumina covariates SequencesWithQuality dataset genotypes macOS Pielou's SampleData denoised osx linux EMP linenos unifrac UniFrac univariate differentially ASVs yml env denoise demultiplexing shannon demuliplexed